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Product Name
GPX3 antibody
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Description
GPX3 Rabbit Polyclonal antibody. Positive WB detected in mouse kidney tissue, HEK-293 cells, human placenta tissue. Positive IF detected in HEK-293 cells. Observed molecular weight by Western-blot: 20-27 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Glutathione peroxidase 3 antibody; GPx 3 antibody; GPx P antibody; GPX3 antibody; GPXP antibody; GSHPx 3 antibody; GSHPx P antibody; Plasma glutathione peroxidase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GPX3 recombinant protein (Accession Number: NM_002084). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:111148(GPX3 Antibody) at dilution of 1:600
Immunofluorescent analysis of (-20oc Ethanol) fixed HEK-293 cells using Catalog No:111148(GPX3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GPX3 (glutathione peroxidase 3) is also named as Plasma Glutathione Peroxidase. GPX3 can protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPX3 is secreted into plasma and like GPX1, is an 100 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. It can be detected a band of 20 kDa which is considered as the chain A of GPX3 (selenocysteine to glycine mutant).
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