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Product Name
GPT2 antibody
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Description
GPT2 Rabbit Polyclonal antibody. Positive WB detected in mouse kidney tissue, human skeletal muscle tissue. Positive IP detected in mouse kidney tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 58 kDa
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Tested applications
ELISA, IHC, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AAT2 antibody; Alanine aminotransferase 2 antibody; ALT2 antibody; GPT 2 antibody; GPT2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GPT2 recombinant protein (Accession Number: NM_133443). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111147(GPT2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111147(GPT2 antibody) at dilution of 1:100 (under 40x lens)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:111147(GPT2 Antibody) at dilution of 1:600
IP Result of anti-GPT2 (IP:Catalog No:111147, 4ug; Detection:Catalog No:111147 1:500) with mouse kidney tissue lysate 7200ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:111147(GPT2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GPT2 represents glutamic pyruvate transaminase (alanine aminotransferase) 2
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