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Product Name
GPT Polyclonal Antibody
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Description
Polyclonal antibody to GPT
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
GPT antibody; AAT1 antibody; ALT1 antibody; GPT1 antibody; alanine aminotransferase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human GPT (NP_005300.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:1000
IHC 1:50 - 1:100 -
Validations
Western blot - GPT Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GPT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - GPT Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using GPT antibody at dilution of 1:100 (40x lens).
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Background
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"