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Product Name
GPRC5A Polyclonal Antibody
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Description
Polyclonal antibody to GPRC5A
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
GPRC5A antibody; GPCR5A antibody; PEIG-1 antibody; RAI3 antibody; RAIG1 antibody; TIG1 antibody; retinoic acid-induced protein 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 258-357 of human GPRC5A (NP_003970.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - GPRC5A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GPRC5A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunohistochemistry - GPRC5A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using GPRC5A antibody at dilution of 1:100 (40x lens).
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Background
Orphan receptor. Could be involved in modulating differentiation and maintaining homeostasis of epithelial cells. This retinoic acid-inducible GPCR provide evidence for a possible interaction between retinoid and G-protein signaling pathways. Functions as a negative modulator of EGFR signaling (By similarity). May act as a lung tumor suppressor.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"