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Product Name
GPR78 Polyclonal Antibody
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Description
Polyclonal antibody to GPR78
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
GPR78 antibody; G-protein coupled receptor 78 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 294-363 of human GPR78 (NP_543009.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - GPR78 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GPR78 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - GPR78 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human placenta using GPR78 antibody at dilution of 1:100 (40x lens).
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Background
Orphan receptor. Displays a significant level of constitutive activity. Its effect is mediated by G(s)-alpha protein that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"