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GPR50 antibody

Cat.#: 111129

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Product Information

  • Product Name
    GPR50 antibody
  • Documents
  • Description
    GPR50 Rabbit Polyclonal antibody. Positive FC detected in K-562 cells. Positive IF detected in SH-SY5Y cells, mouse Hipp℃amus tissue. Positive WB detected in transfected cells.
  • Tested applications
    ELISA, IF, WB, FC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    G protein coupled receptor 50 antibody; GPR50 antibody; Mel1c antibody; Melatonin related receptor antibody
  • Immunogen
    Human GPR50 Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of GPR50 recombinant protein (Accession Number: NM_004224). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: N/AIF: N/A

  • Validations

    WB result of GPR50 antibody (Catalog No:111129, 1:5,000) by Dr. Qian Li, University of Edinburgh. U1: Lysates from SH-SY5Y cells, untransfected, no endogenous GPR50, negative control,15ug; G1: Lysates from HEK293 cells, with transfected GPR50, positive control, 2ug; G2: Lysates from HEK293 cells, with transfected GPR50, 10ug; U2: Lysates from HEK293 cells, untransfected, with endogenous GPR50, 10ug; U3: Lysates from HEK293 cells, untransfected, with endogenous GPR50, 10ug. Optimal dilution: 1 in 5000, Signal detection: ECL, Pierce; Exposure time: 10min.

    WB result of GPR50 antibody (Catalog No:111129, 1:5,000) by Dr. Qian Li, University of Edinburgh. U1: Lysates from SH-SY5Y cells, untransfected, no endogenous GPR50, negative control,15ug; G1: Lysates from HEK293 cells, with transfected GPR50, positive control, 2ug; G2: Lysates from HEK293 cells, with transfected GPR50, 10ug; U2: Lysates from HEK293 cells, untransfected, with endogenous GPR50, 10ug; U3: Lysates from HEK293 cells, untransfected, with endogenous GPR50, 10ug. Optimal dilution: 1 in 5000, Signal detection: ECL, Pierce; Exposure time: 10min.

    IF result of GPR50 antibody (Catalog No:111129, 1:1,000) with SH-SY5Y cells transfected with GPR50 by Dr. Qian Li, University of Edinburgh.

    IF result of GPR50 antibody (Catalog No:111129, 1:1,000) with SH-SY5Y cells transfected with GPR50 by Dr. Qian Li, University of Edinburgh.

    IF result of GPR50 antibody (Catalog No:111129, 1:50) with mouse hippocampus by Dr. Qian Li, University of Edinburgh.

    IF result of GPR50 antibody (Catalog No:111129, 1:50) with mouse hippocampus by Dr. Qian Li, University of Edinburgh.

    1X10^6 K-562 cells were stained with 0.2ug GPR50 antibody (Catalog No:111129, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 K-562 cells were stained with 0.2ug GPR50 antibody (Catalog No:111129, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    GPR50 represents G protein-coupled receptor 50

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"