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Product Name
GPR37/Pael-R antibody
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Description
GPR37/Pael-R Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 50-55kd
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EDNRBL antibody; ETBR LP 1 antibody; GPR37 antibody; hET(B)R LP antibody; PAELR antibody; Pael-R antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GPR37/Pael-R recombinant protein (Accession Number: NM_005302). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111127(GPR37 antibody) at dilution of 1:1600
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Background
The orphan G protein-coupled receptor GPR37, also known as PAELR (parkin-associated endothelin receptor-like receptor) or ETBR-LP-1 (endothelin B receptor-like protein 1), is a 613 aa, multi-pass membrane protein predominantly expressed in the brain. It is a substrate of parkin (PARK2). When overexpressed in cells, GPR37 tends to become insoluble, unfolded, and ubiquitinated in vivo. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease (JPD). This antibody recognizes endogenous GPR37, which migrates as an approximately 50-55 kDa band in SDS-PAGE (PMID:17519329).
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References
- Meyer RC, Giddens MM, Schaefer SA, Hall RA. GPR37 and GPR37L1 are receptors for the neuroprotective and glioprotective factors prosaptide and prosaposin. Proceedings of the National Academy of Sciences of the United States of America. 110(23):9529-34. 2013.
- Lundius EG, Vukojevic V, Hertz E. GPR37 protein trafficking to the plasma membrane regulated by prosaposin and GM1 gangliosides promotes cell viability. The Journal of biological chemistry. 289(8):4660-73. 2014.
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