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Product Name
GPR35 antibody
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Description
GPR35 Rabbit Polyclonal antibody. Positive FC detected in A549 cells. Positive WB detected in A549 cells, human brain tissue, human stomach tissue. Observed molecular weight by Western-blot: 30-34 kDa
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Tested applications
ELISA, WB, FC
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Species reactivity
Human; other species not tested.
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Alternative names
G protein coupled receptor 35 antibody; GPR35 antibody; KYNA receptor antibody; Kynurenic acid receptor antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005301). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:111126(GPR35 antibody) at dilution of 1:600
1X10^6 A549 cells were stained with 0.2ug GPR35 antibody (Catalog No:111126, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
GPR35, also named as KYNA receptor, belongs to the G-protein coupled receptor 1 family. GPR35 acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of GPR35 is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins (PMID:16754668). This antibody is specific to GPR35.
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