-
Product Name
GPI Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to GPI
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
GPI antibody; AMF antibody; GNPI antibody; NLK antibody; PGI antibody; PHI antibody; SA-36 antibody; SA36 antibody; glucose-6-phosphate isomerase antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human GPI (NP_000166.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - GPI Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GPI antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - GPI Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using GPI antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - GPI Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using GPI antibody at dilution of 1:200 (40x lens).
Immunofluorescence - GPI Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using GPI antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"