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Product Name
GPAM Polyclonal Antibody
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Description
Polyclonal antibody to GPAM
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Tested applications
WB, IHC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
GPAM antibody; GPAT antibody; GPAT1 antibody; glycerol-3-phosphate acyltransferase, mitochondrial antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 594-828 of human GPAM (NP_001231878.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200 -
Validations
Western blot - GPAM Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GPAM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - GPAM Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using GPAM antibody at dilution of 1:100 (40x lens).
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Background
Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"