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Product Name
GP9 Polyclonal Antibody
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Description
Polyclonal antibody to GP9
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
GP9 antibody; CD42a antibody; GPIX antibody; platelet glycoprotein IX antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 17-147 of human GP9 (NP_000165.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:20 - 1:100 -
Validations
Western blot - GP9 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GP9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - GP9 Polyclonal Antibody
Immunofluorescence analysis of A-549 cells using GP9 antibody . Blue: DAPI for nuclear staining.
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Background
The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis. GP-IX may provide for membrane insertion and orientation of GP-Ib.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"