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Product Name
GP73/GOLPH2 antibody
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Description
GP73/GOLPH2 Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human liver cancer tissue, human heart tissue, human kidney tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive IF detected in Hela cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 73 kDa
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Tested applications
ELISA, WB, IF, IHC, FC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
golgi protein 73kD antibody; GOLM1 antibody; GOLPH2 antibody; GP73 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GP73/GOLPH2 recombinant protein (Accession Number: NM_016548). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:111054(GOLM1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:111054(GOLM1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using GOLM1 antibody Catalog No:111054 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:111054(GOLM1 antibody) at dilution of 1:1500
IP Result of anti-GOLM1 (IP:Catalog No:111054, 4ug; Detection:Catalog No:111054 1:1000) with HeLa cells lysate 2800ug.
1X10^6 HeLa cells were stained with 0.2ug GOLM1 antibody (Catalog No:111054, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
GOLM1 represents golgi membrane protein 1
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References
- Bao YX, Cao Q, Yang Y. Expression and prognostic significance of golgiglycoprotein73 (GP73) with epithelial-mesenchymal transition (EMT) related molecules in hepatocellular carcinoma (HCC). Diagnostic pathology. 8:197. 2013.
- Wang T, Liu H, Wang Y, Liu C, Sun X. RCAN1 increases Aβ generation by promoting N-glycosylation via oligosaccharyltransferase. Current Alzheimer research. 11(4):332-9. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"