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Product Name
GORASP1 Polyclonal Antibody
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Description
Polyclonal antibody to GORASP1
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
GORASP1 antibody; GOLPH5 antibody; GRASP65 antibody; P65 antibody; Golgi reassembly-stacking protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 221-440 of human GORASP1 (NP_114105.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100 -
Validations
Western blot - GORASP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GORASP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - GORASP1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using GORASP1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - GORASP1 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using GORASP1 antibody . Blue: DAPI for nuclear staining.
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Background
Plays an important role in assembly and membrane stacking of the Golgi cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes. Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"