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Product Name
GOLIM4 Polyclonal Antibody
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Description
Polyclonal antibody to GOLIM4
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
GOLIM4 antibody; GIMPC antibody; GOLPH4 antibody; GPP130 antibody; P138 antibody; Golgi integral membrane protein 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 34-300 of human GOLIM4 (NP_055313.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - GOLIM4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GOLIM4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"