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Product Name
GNAT1 antibody
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Description
GNAT1 Rabbit Polyclonal antibody. Positive WB detected in mouse eye tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 40-45 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CSNBAD3 antibody; GBT1 antibody; GNAT1 antibody; GNATR antibody; Transducin alpha 1 chain antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000172). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
WB results of GNAT1 antibody (Catalog No:110993) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine.
mouse eye tissue were subjected to SDS PAGE followed by western blot with Catalog No:110993(GNAT1 antibody) at dilution of 1:300
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:110993(GNAT1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GNAT1, also named as GNATR, belongs to the G-alpha family and G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3). This antibody is specific to GNAT1.
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