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Product Name
GNAI2 antibody
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Description
GNAI2 Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human tonsillitis tissue, human brain tissue, human kidney tissue, human lung cancer tissue. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 35-40 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GIP antibody; GNAI2 antibody; GNAI2B antibody; H_LUCA15.1 antibody; H_LUCA16.1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GNAI2 recombinant protein (Accession Number: XM_047447979). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110988(GNAI2 antibody) at dilution of 1:800
Immunofluorescent analysis of Hela cells, using GNAI2 antibody Catalog No:110988 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-GNAI2 (IP:Catalog No:110988, 4ug; Detection:Catalog No:110988 1:500) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:110988(GNAI2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:110988(GNAI2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.
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References
- Li J, Zhu S, Kozono D. Genome-wide shRNA screen revealed integrated mitogenic signaling between dopamine receptor D2 (DRD2) and epidermal growth factor receptor (EGFR) in glioblastoma. Oncotarget. 5(4):882-93. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"