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Product Name
GMPS Polyclonal Antibody
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Description
Polyclonal antibody to GMPS
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Tested applications
WB, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
GMPS antibody; GATD7 antibody; guanine monophosphate synthase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 394-693 of human GMPS (NP_003866.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:50 - 1:100 -
Validations
Western blot - GMPS Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GMPS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunoprecipitation - GMPS Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug GMPS antibody . Western blot was performed from the immunoprecipitate using GMPS antibody at a dilition of 1:1000.
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Background
Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"