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Product Name
Glypican 1 antibody
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Description
Glypican 1 Rabbit Polyclonal antibody. Positive IF detected in BxPC-3 cells. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in NIH/3T3 cells, BxPC-3 cells, HepG2 cells. Positive IP detected in BxPC-3 cells. Observed molecular weight by Western-blot: 55 kDa, 80 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ38078 antibody; glypican antibody; Glypican 1 antibody; GPC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Glypican 1 recombinant protein (Accession Number: NM_002081). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:50-1:500
IF: 1:10-1:100
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111087(GPC1 antibody) at dilution of 1:300
IP Result of anti-GPC1 (IP:Catalog No:111087, 4ug; Detection:Catalog No:111087 1:500) with BxPC-3 cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:111087(GPC1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:111087(GPC1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of BxPC-3 cells using Catalog No:111087(GPC1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Glypicans are a family of heparin sulfate proteoglycans (HSPGs) that are attached to the cell membrane by a glycosyl-phosphatidylinositol anchor. As heparin sulfate-carrying molecules, glypicans are considered to have the ability to modulate the activities of heparin-binding growth factors (HBGFs). The expression of glypicans is regulated temporally and spatially during development, suggesting they are involved in development and morphogenesis. To date, six glypicans (GPC1 to GPC6) have been identified in mammals. GPC1 is most ubiquitously expressed in adult tissues. GPC1 promotes efficient signaling by HBGFs and plays a critical role in cell growth and differentiation. GPC1 is involved in tumorigenesis and angiogenesis, and is frequently overexpressed in several types of tumors including pancreatic carcinoma, breast cancer and glioma.
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References
- Frolova EG, Drazba J, Krukovets I. Control of organization and function of muscle and tendon by thrombospondin-4. Matrix biology : journal of the International Society for Matrix Biology. 37:35-48. 2014.
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