Gluten Exorphin A5 peptide

Gluten Exorphin A5 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GYYPT

H-Gly-Tyr-Tyr-Pro-Thr-OH

5

97.8%

C₂₉H₃₇N₅O₉

599.64

142155-24-6

Synthetic

Powder

The sequence of this opioid peptide was found at 15 sites in the primary structure of the high molecular weight glutenin. Gluten exorphin A5 is highly specific for δ-receptors and displays opioid activity in the MVD assay. The N-terminal Gly increases its activity - thus the structure-activity relationships of gluten exorphins A are quite different from those of the endogenous opioid peptides.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• S.Fukudome and M.Yoshikawa, FEBS Lett., 296, 107 (1992)
• S.Fukudome et al., Peptide Chemistry 1991, A.Suzuki, ed., Protein Research Foundation, Osaka, p. 345, (1992)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.