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Product Name
GLUT3 antibody
- Documents
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Description
GLUT3 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, HEK-293 cells, HeLa cells, HepG2 cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 48kd
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
GLUT 3 antibody; GLUT3 antibody; SLC2A3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GLUT3 recombinant protein (Accession Number: NM_006931). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Immunohistochemistry of paraffin-embedded human brain slide using Catalog No:111078(SLC2A3 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human brain slide using Catalog No:111078(SLC2A3 Antibody) at dilution of 1:50
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:111078(SLC2A3 Antibody) at dilution of 1:600
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Background
GLUT3 (SLC2A3) belongs to the glucose transporter family (GLUTs) which mediates the transport of glucose across cellular membranes in mammalian cells. There are three subclasses within GLUTs: class I comprises the classical transporters GLUT1-4 and GLUT14; class II contains the "odd" isoforms GLUT5, 7, 9, and 11; the isoforms GLUT6, 8, 10, 12 and the proton driven myoinositol transporter HMIT (GLUT13) belong to class III. GLUT3 is considered as a neuron-specific glucose transporter because of its dominant expression in the brain in various species. However, besides the brain GLUT3 is also expressed in tissues with high demand for glucose such as sperm, preimplantation embryos, circulating white blood cells, and an array of carcinoma cell lines.Recently GLUT3 has been identified as a sensitive and specific marker for embyonal carcinomas and yolk tumors.The GLUT14 is believed to be the duplicate gene of GLUT3 given to the high identity in sequence between them. This antibody was generated against the internal region of human GLUT3 and may cross-react with GLUT14. (23343953)
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References
- Huang D, Li T, Li X. HIF-1-mediated suppression of acyl-CoA dehydrogenases and fatty acid oxidation is critical for cancer progression. Cell reports. 8(6):1930-42. 2014.
- Fang F, Chen D, Yu P. Effects of Bisphenol A on glucose homeostasis and brain insulin signaling pathways in male mice. General and comparative endocrinology. 212:44-50. 2015.
- Inoue M, Hur JY, Kihara T. Human brain proteins showing neuron-specific interactions with γ-secretase. The FEBS journal. 282(14):2587-99. 2015.
- Cho MH, Park CK, Park M, Kim WK, Cho A, Kim H. Clinicopathologic Features and Molecular Characteristics of Glucose Metabolism Contributing to ¹⁸F-fluorodeoxyglucose Uptake in Gastrointestinal Stromal Tumors. PloS one. 10(10):e0141413. 2015.
- Fang F, Gao Y, Wang T. Insulin signaling disruption in male mice due to perinatal bisphenol A exposure: Role of insulin signaling in the brain. Toxicology letters. 245:59-67. 2016.
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