Product Name

GLUT1 antibody

Documents

Description

GLUT1 Rabbit Polyclonal antibody. Positive WB detected in Y79 cells, HEK-293 cells, NIH/3T3 cells, SH-SY5Y cells. Positive IHC detected in human breast cancer tissue, human lung cancer tissue. Positive IF detected in HeLa cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 45-55kd

Tested applications

ELISA, WB, IHC, FC, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

DYT17 antibody; DYT18 antibody; GLUT antibody; GLUT 1 antibody; GLUT1 antibody; HepG2 glucose transporter antibody; PED antibody; SLC2A1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of GLUT1 recombinant protein (Accession Number: NM_006516). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111076(SLC2A1,GLUT1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:111076(SLC2A1,GLUT1 antibody) at dilution of 1:50 (under 40x lens)

Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:111076(SLC2A1,GLUT1 antibody) at dilution of 1:1000

1X10^6 HeLa cells were stained with 0.2ug SLC2A1,GLUT1 antibody (Catalog No:111076, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Immunofluorescent analysis of HeLa cells using Catalog No:111076(SLC2A1,GLUT1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

SLC2A1, also known as GLUT1, is an ubiquitously expressed glucose transporter and responsible for the basal level of glucose uptake in most cell types. Human erythrocytes express the highest level of the SLC2A1. Defects in SLC2A1 are the cause of GLUT1 deficiency syndrome type 1 and type 2. High expression of SLC2A1 has been reported to be a reliable immunohistochemical marker for juvenile hemangiomas.

References

Lai G, Wu J, Liu X, Zhao Y. 20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway. Molecular endocrinology (Baltimore, Md.). 26(11):1907-16. 2012.
Zhao W, Chang C, Cui Y. Steroid receptor coactivator-3 regulates glucose metabolism in bladder cancer cells through coactivation of hypoxia inducible factor 1α. The Journal of biological chemistry. 289(16):11219-29. 2014.
Huang D, Li T, Li X. HIF-1-mediated suppression of acyl-CoA dehydrogenases and fatty acid oxidation is critical for cancer progression. Cell reports. 8(6):1930-42. 2014.
Ma X, Li C, Sun L. Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Nature communications. 5:5212. 2014.
Fang F, Chen D, Yu P. Effects of Bisphenol A on glucose homeostasis and brain insulin signaling pathways in male mice. General and comparative endocrinology. 212:44-50. 2015.
Teng CF, Hsieh WC, Wu HC. Hepatitis B Virus Pre-S2 Mutant Induces Aerobic Glycolysis through Mammalian Target of Rapamycin Signal Cascade. PloS one. 10(4):e0122373. 2015.
Luo J, Wu N, Jiang B. Marine Bromophenol Derivative 3,4-Dibromo-5-(2-bromo-3,4-dihydroxy-6-isopropoxymethyl benzyl)benzene-1,2-diol Protects Hepatocytes from Lipid-Induced Cell Damage and Insulin Resistance via PTP1B Inhibition. Marine drugs. 13(7):4452-69. 2015.
Fang F, Gao Y, Wang T. Insulin signaling disruption in male mice due to perinatal bisphenol A exposure: Role of insulin signaling in the brain. Toxicology letters. 245:59-67. 2016.

GLUT1 antibody

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