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Product Name
GLUT1 antibody
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Description
GLUT1 Mouse Monoclonal antibody. Positive WB detected in HEK-293 cells, NIH/3T3 cells. Positive FC detected in Jurkat cells. Positive IHC detected in human lung cancer tissue. Observed molecular weight by Western-blot: 45-55 kDa
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Tested applications
ELISA, IHC, WB, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DYT17 antibody; DYT18 antibody; GLUT antibody; GLUT 1 antibody; GLUT1 antibody; HepG2 glucose transporter antibody; PED antibody; SLC2A1 antibody; SLC2A1 antibody; GLUT1 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of GLUT1 recombinant protein (Accession Number: NM_006516). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107300(SLC2A1,GLUT1 Antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using (SLC2A1,GLUT1 Antibody) at dilution of 1:300 (under 10x lens).
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using (SLC2A1,GLUT1 Antibody) at dilution of 1:300 (under 40x lens).
1X10^6 Jurkat cells were stained with 0.2ug SLC2A1,GLUT1 antibody (Catalog No:107300, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.
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Background
GLUT1, also known as SLC2A1, is an ubiquitously expressed glucose transporter and responsible for the basal level of glucose uptake in most cell types. Human erythrocytes express the highest level of the GLUT1. Defects in SLC2A1 are the cause of GLUT1 deficiency syndrome type 1 and type 2. High expression of GLUT1 has been reported to be a reliable immunohistochemical marker for juvenile hemangiomas.
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