Glucagon (19-29) (human, rat, porcine) peptide

Glucagon (19-29) (human, rat, porcine) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

AQDFVQWLMNT

H-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-OH

11

97.3%

C₆₁H₈₉N₁₅O₁₈S

1352.53

Synthetic

Powder

This glucagon fragment inhibited both the Ca2+ activated and Mg2+ dependent ATPase activity and Ca2+ transport in liver plasma membranes with an efficiency 1000-fold higher than that of glucagon. It is likely to be the active peptide involved in the inhibition of the liver Ca2+ pump.Miniglucagon, the ultimate processing product of proglucagon, is a regulator of the pancreatic islet physiology.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• A.Mallat et al., Nature, 325, 620 (1987)
• S.Dalle et al., J. Biol. chem., 274, 10864 (1999)
• S.Dalle et al., Diabetes, 51, 406 (2002)
• D.Bataille et al., Ann. NY Acad. Sci., 1070, 161 (2006)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.