(Glu2)-TRH peptide

(Glu2)-TRH peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

pGlu-EP

pGlu-Glu-Pro-NH2

3

96.4%

C₁₅H₂₂N₄O₆

354.36

Synthetic

Powder

This TRH analog, also called fertilization-promoting peptide (FPP), was previously purified and characterized from both rabbit prostate and human semen. Nanomolar concentrations of this peptide have been shown to enhance capacitation of mouse epididymal spermatozoa with a concomitant increase in fertilizing ability. It was also detected in the thyroid gland.According to a study of Nguyen et al. Pyr-Glu-Pro-NH2 opposes the cholinergic effect of TRH in the mammalian CNS Co-perfusi

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• S.M.Cockle et al., Biochim. Biophys. Acta, 1227, 60 (1994)
• R.J.Ashworth et al., J. Endocrinol., 142, 111 (1994)
• P.J.Gkonos et al., Peptides, 15, 1281 (1994)
• C.M.Green et al., Mol. Reprod. Dev., 38, 215 (1994)
• A.Cremandes et al., Eur. J. Pharmacol., 358, 63 (1998)
• K.Prokai-Tatrai et al., Bioorg. Med. Chem. Lett., 13, 1011 (2003)
• V.Nguyen et al., Neurosci. Lett., 415, 64 (2007)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.