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Product Name
GLS2 Polyclonal Antibody
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Description
Polyclonal antibody to GLS2
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
GLS2 antibody; GA antibody; GLS antibody; LGA antibody; hLGA antibody; glutaminase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human GLS2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IF 1:50 - 1:200 -
Validations
Western blot - GLS2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GLS2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"