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Product Name
GlnRS antibody
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Description
GlnRS Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IHC detected in human prostate cancer tissue. Positive IP detected in PC-3 cells. Positive WB detected in PC-3 cells. Observed molecular weight by Western-blot: 88 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GlnRS antibody; Glutamine tRNA ligase antibody; glutaminyl tRNA synthetase antibody; PRO2195 antibody; QARS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GlnRS recombinant protein (Accession Number: BC016634). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111026(QARS antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111026(QARS antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-QARS (IP:Catalog No:111026, 4ug; Detection:Catalog No:111026 1:500) with PC-3 cells lysate 3200ug.
Immunofluorescent analysis of PC-3 cells using Catalog No:111026(QARS Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GLnRS(glutaminyl-tRNA synthetase) is a class I aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The specificity of this reaction determines the fidelity of mRNA translation. At least 1 synthetase exists in the cytoplasm for each amino acid.GlnRS is present in eukaryotes but is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln)(PMID:8078941).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"