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Product Name
Human GDNF recombinant protein
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Description
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
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Protein name
Glial cell line-derived neurotrophic factor
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Protein short names
HFB1-GDNF; ATF2; AI385739; ASTROCYTE-DERIVED TROPHIC FACTOR; NEUROTROPHIC FACTOR; HSCR3; GLIAL CELL LINE-DERIVED NEUROTROPHIC FACTOR; ATF1; MGDNF; ATF
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Uniprot ID
P39905
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Gene Name
GDNF
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mature form of human GDNF (P39905-2) (Arg 83-Ile 185) was expressed and purified, with additional two aa (Gly& Pro) at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human GDNF consists of 105 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
1. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-15 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 His Chimera.
2. Measured by its ability to bind biotinylated Human GFRa1-His in functional ELISA. -
Validations
Human GDNF Protein SDS-PAGE
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