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Product Name
Gli2 Polyclonal Antibody
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Description
Polyclonal antibody to Gli2
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Tested applications
WB, IF, FC
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Species reactivity
Human
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Alternative names
GLI2 antibody; CJS antibody; HPE9 antibody; PHS2 antibody; THP1 antibody; THP2 antibody; zinc finger protein GLI2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Gli2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IF 1:20 - 1:50
FC 1:20 - 1:50 -
Validations
Western blot - Gli2 Polyclonal Antibody
Western blot analysis of extracts of Jurkat cells, using Gli2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Functions as transcription regulator in the hedgehog (Hh) pathway. Functions as transcriptional activator. May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development.; Isoform 1, isoform 2, isoform 3 and isoform 4: Act as transcriptional activators in T-cell leukemia virus type 1 (HTLV-1)-infected cells in a Tax-dependent manner. Bind to the DNA sequence 5'-GAACCACCCA-3' which is part of the Tax-responsive element (TRE-2S) regulatory element that augments the Tax-dependent enhancer of HTLV-1. Are involved in the smoothened (SHH) signaling pathway.; Isoform 5: Acts as a transcriptional repressor.
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