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Product Name
GHRL antibody
- Documents
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Description
GHRL Rabbit Polyclonal antibody. Positive WB detected in human stomach tissue. Positive IHC detected in human stomach tissue, mouse stomach tissue, rat stomach tissue. Observed molecular weight by Western-blot: 13 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
Appetite regulating hormone antibody; Ghrelin Obestatin antibody; GHRL antibody; Growth hormone secretagogue antibody; Motilin related peptide antibody; MTLRP antibody; obestatin antibody; Protein M46 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GHRL recombinant protein (Accession Number: NM_001302825). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human stomach tissue were subjected to SDS PAGE followed by western blot with Catalog No:110968(GHRL antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human stomach using Catalog No:110968(GHRL antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach using Catalog No:110968(GHRL antibody) at dilution of 1:100 (under 40x lens)
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Background
GHRL encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is predominantly produced in endocrine cells in the gastric mucosa, called X/A-like or ghrelin cells, from where it is secreted into the plasma. In the pituitary gland, ghrelin stimulates growth hormone release and regulates food intake and energy metabolism. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes.
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