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GFP tag antibody

Cat.#: 110950

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Product Information

  • Product Name
    GFP tag antibody
  • Documents
  • Description
    GFP tag Rabbit Polyclonal antibody. Positive IP detected in Transfected HEK-293 cells. Positive WB detected in recombinant protein. Positive IF detected in Transfected HEK-293 cells. Observed molecular weight by Western-blot: 30 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Tag
  • Alternative names
    CFP antibody; eGFP antibody; eYFP antibody; GFP antibody; GFP tag antibody; YFP antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of GFP tag recombinant protein (Accession Number: AVU73901). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:2000-1:10000

    IP: 1:2000-1:20000

    IF: 1:20-1:200

  • Validations

    Western blot of eGFP-tagged fusion protein with anti-eGFP-tag (Catalog No:117319) at various dilutions.

    Western blot of eGFP-tagged fusion protein with anti-eGFP-tag (Catalog No:117319) at various dilutions.

    IP Result of anti-GFP tag (IP:Catalog No:117319, 4ug; Detection:Catalog No:117319 1:5000) with Transfected HEK-293 cells lysate 300ug.

    IP Result of anti-GFP tag (IP:Catalog No:117319, 4ug; Detection:Catalog No:117319 1:5000) with Transfected HEK-293 cells lysate 300ug.

    Immunofluorescent analysis of (-20oc Ethanol) fixed Transfected HEK-293 cells using Catalog No:117319(GFP tag Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed Transfected HEK-293 cells using Catalog No:117319(GFP tag Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzyme activity of HRP.
  • References
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    • Zhang Y, Ning JF, Qu XQ, Meng XL, Xu JP. TAT-mediated oral subunit vaccine against white spot syndrome virus in crayfish. Journal of virological methods. 181(1):59-67. 2012.
    • Li D, Wang M. Construction of a bicistronic vector for the co-expression of two genes in Caenorhabditis elegans using a newly identified IRES. BioTechniques. 52(3):173-6. 2012.
    • Wen Y, Lan J, Huang H. Application of eGFP to label human periodontal ligament stem cells in periodontal tissue engineering. Archives of oral biology. 57(9):1241-50. 2012.
    • Zhou J, Chen H, Li S. Fibroblastic potential of CD41+ cells in the mouse aorta-gonad-mesonephros region and yolk sac. Stem cells and development. 21(14):2592-605. 2012.
    • Li WP, Xia LQ, Ding XZ. Expression and characterization of a recombinant Cry1Ac crystal protein fused with an insect-specific neurotoxin ω-ACTX-Hv1a in Bacillus thuringiensis. Gene. 498(2):323-7. 2012.
    • Wen Y, Wang F, Zhang W. Application of induced pluripotent stem cells in generation of a tissue-engineered tooth-like structure. Tissue engineering. Part A. 18(15-16):1677-85. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"