-
Product Name
GEMIN5 antibody
- Documents
-
Description
GEMIN5 Rabbit Polyclonal antibody. Positive IHC detected in human cerebellum tissue, human cervical cancer tissue. Positive IF detected in HeLa cells. Positive IP detected in HeLa cells. Positive WB detected in Jurkat cells, HEK-293 cells, HeLa cells. Observed molecular weight by Western-blot: 168 kDa
-
Tested applications
ELISA, WB, IP, IF, IHC
-
Species reactivity
Human; other species not tested.
-
Alternative names
DKFZp586M1824 antibody; Gem assOCiated protein 5 antibody; GEMIN5 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of GEMIN5 recombinant protein (Accession Number: NM_015465). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:110939(GEMIN5 Antibody) at dilution of 1:1500
IP Result of anti-GEMIN5 (IP:Catalog No:110939, 5ug; Detection:Catalog No:110939 1:1000) with HeLa cells lysate 220ug.
Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using Catalog No:110939(GEMIN5 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:110939(GEMIN5 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
-
Background
GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"