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Product Name
GEFT antibody
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Description
GEFT Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, Transfected HEK-293 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 64 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GEFT antibody; p63RhoGEF antibody; RhoA/RAC/CDC42 exchange factor antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GEFT recombinant protein (Accession Number: NM_133483). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110934(GEFT antibody) at dilution of 1:300
IP Result of anti-GEFT (IP:Catalog No:110934, 4ug; Detection:Catalog No:110934 1:400) with HEK-293 cells lysate 2000ug.
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:110934(GEFT Antibody) at dilution of 1:1000
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Background
GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis.
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