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Product Name
GEFT antibody
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Description
GEFT Mouse Monoclonal antibody. Positive WB detected in mouse skeletal muscle tissue, human brain tissue, mouse brain tissue, rat brain tissue, rat skeletal muscle tissue. Positive IHC detected in human heart tissue, human brain tissue. Observed molecular weight by Western-blot: 53 kDa, 63 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GEFT antibody; p63RhoGEF antibody; RhoA/RAC/CDC42 exchange factor antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of GEFT recombinant protein (Accession Number: NM_133483). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:107293(GEFT antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human heart using Catalog No:107293(GEFT antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:107293(GEFT antibody) at dilution of 1:50 (under 40x lens)
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Background
GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"