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  • GE11 peptide

GE11 peptide

For research use only. We do not sell to patients.

Cat.#: 318896

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Optional Service: TFA RemovalWhat's this?

Special Price 94.5 USD

Availability: 1-2 weeks
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Product Information

  • Product Name
    GE11 peptide
  • Documents
  • Quantity/Unit
    1 Vial
  • Sequence
    H-YHWYGYTPQNVI-OH
  • Three letter code
    H-Tyr-His-Trp-Tyr-Gly-Tyr-Thr-Pro-Gln-Asn-Val-Ile-OH
  • Length (aa)
    12
  • Peptide Purity (HPLC)
    95.2
  • Molecular Formula
    C75H97N17O19
  • Molecular Weight
    1540.67
  • Source
    Synthetic
  • Additional Information
    EGFR is a crucial driver of tumorigenesis abundantly overexpressed, amplified or mutated in many cancers including lung and breast cancers, and glioblastomas. EGFR overexpression or mutation often correlate with drug resistance thereby presenting EGFR as a possible target in many malignant tumors. For the identification of EGFR binding peptides, T7 phage displayed peptide library was incubated with the purified human EGFR. After three rounds of panning, the dodecapeptide termed GE11 (YHWYGYTPQNVI) was shown to bind specifically to EGFR. The peptide showed high binding affinity in nanomolar range (KD = approximately 22 nM) that could be inhibited by EGF, indicating that they bound to the same site on EGFR. In vivo targeting ability of the radioactively labeled 125I-GE11 peptide was validated after intravenous (i.v.) infusion of the conjugate into nude mice bearing subcutaneous hepatocellular carcinoma (SMMC-7721) xenografts. The tumor targeting of 125I-GE11 was inhibited by a 100-fold excess of cold GE11 peptide. In addition, GE11 peptide was used as a gene delivery vehicle upon conjugation to the polyethylenimine (PEI) polyplexes using a glycine (G-G-G-S) linker system and intravenously administered to the SMMC-7721 bearing mice. Cheng and colleagues reported the conjugation of GE11 to Doxil for targeted in vivo delivery to EGFR-overexpressing non-small cell lung cancer (A549). Doxil (PEGylated liposomal Dox, PEG-LP/Dox, or non-PEGylated Dox, LP/Dox) is an FDA approved chemotherapy comprising doxorubicin drug encapsulated in liposomes. Doxil, also referred to as Lipo-Dox, is widely used in the clinics for ovarian cancer treatment. Very promising results were obtained when the GE11-conjugated doxorubicin filled liposomes (GE11-PS-Dox) were used to treat ovarian cancer xenograft bearing mice, indicating GE11-guided drug targeting as an alternative for treatment of EGFR overexpressing ovarian cancers. GE11-PS-Dox treatment very significantly increased the survival rate with minimal toxicity compared to non-targeting control (PS-Dox) or the clinically used Lipo-Dox. In a similar fashion, GE11-PS-Dox also showed prolonged survival compared to the PBS- and non-targeting treatment controls (PS-Dox, Lipo-Dox) in a preclinical liver cancer treatment study. Moreover, several GE11-targeted radionuclides have been studied in imaging of EGFR overexpressing tumors with either PET or SPECT.
  • Storage Guidelines
    Ideally GE11 peptide should be stored in a freezer at or below -9C. GE11 peptide should be refrigerated after reconstitution. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
  • References
    • Li Z, Zhao R, Wu X, Sun Y, Yao M, Li J, et al. Identification and characterization of a novel peptide ligand of epidermal growth factor receptor for targeted delivery of therapeutics. FASEB J. 2005;19(14):1978-85
  • About TFA salt

    Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

    TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

    TFA Removal Service is recommended for:

    • Peptides that will be used in cellular assays
    • Peptides that will be used as APIs or in manufactured products
    • For hydrophilic peptides containing numerous basic residues

Peptide Property

Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"