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Product Name
GDA Polyclonal Antibody
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Description
Polyclonal antibody to GDA
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
GDA antibody; CYPIN antibody; GUANASE antibody; NEDASIN antibody; GAH antibody; guanine deaminase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 165-454 of human GDA (NP_004284.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - GDA Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GDA Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Western blot - GDA Polyclonal Antibody
Western blot analysis of extracts of various cells, using GDA antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunofluorescence - GDA Polyclonal Antibody
Immunofluorescence analysis of A549 cells using GDA antibody . Blue: DAPI for nuclear staining.
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Background
Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"