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Product Name
GCSH antibody
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Description
GCSH Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells, HepG2 cells. Positive IHC detected in human kidney tissue, human brain tissue, human heart tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue, rat ovary tissue. Positive WB detected in HEK-293 cells, HeLa cells, human liver tissue, mouse brain tissue, mouse kidney tissue. Positive IP detected in mouse kidney tissue. Observed molecular weight by Western-blot: 15 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GCE antibody; GCSH antibody; NKH antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GCSH recombinant protein (Accession Number: NM_004483). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:110918(GCSH antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110918(GCSH antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110918(GCSH antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using GCSH antibody Catalog No:110918 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-GCSH (IP:Catalog No:110918, 3ug; Detection:Catalog No:110918 1:1000) with mouse kidney tissue lysate 4000ug.
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Background
GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encoding a precursor protein of 173 amino acids and a mature protein of 125 amino acids.The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).
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References
- Johnson MA, Vidoni S, Durigon R. Amino acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis. PloS one. 9(4):e93597. 2014.
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