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Product Name
GCNT1 Polyclonal Antibody
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Description
Polyclonal antibody to GCNT1
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Tested applications
WB
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Species reactivity
Human
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Alternative names
GCNT1 antibody; C2GNT antibody; C2GNT-L antibody; C2GNT1 antibody; G6NT antibody; NACGT2 antibody; NAGCT2 antibody; glucosaminyl (N-acetyl) transferase 1, core 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-300 of human GCNT1 (NP_001091103.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - GCNT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GCNT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine moiety onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan. Mucin-type core 2 O-glycans play an important role in leukocyte extravasation as they serve as scaffolds for the display of the selectin ligand sialyl Lewis X by leukocytes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"