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Product Name
GCN1L1 Polyclonal Antibody
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Description
Polyclonal antibody to GCN1L1
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
GCN1 antibody; GCN1L antibody; GCN1L1 antibody; PRIC295 antibody; eIF-2-alpha kinase activator GCN1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 2462-2671 of human GCN1L1 (NP_006827.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - GCN1L1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GCN1L1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - GCN1L1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using GCN1L1 antibody at dilution of 1:150 (40x lens).
Immunohistochemistry - GCN1L1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using GCN1L1 antibody at dilution of 1:150 (40x lens).
Immunohistochemistry - GCN1L1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human appendix using GCN1L1 antibody at dilution of 1:150 (40x lens).
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Background
Acts as a positive activator of the EIF2AK4/GCN2 protein kinase activity in response to amino acid starvation. Forms a complex with EIF2AK4/GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of EIF2AK4/GCN2, and hence stimulating EIF2AK4/GCN2 kinase activity. Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator ATF4, by promoting the EIF2AK4/GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52', and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"