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GCK antibody

Cat.#: 110905

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Product Information

  • Product Name
    GCK antibody
  • Documents
  • Description
    GCK Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, mouse pancreas tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 50-52kd
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    GCK antibody; GLK antibody; Glucokinase antibody; glucokinase (hexokinase 4) antibody; Hexokinase 4 antibody; Hexokinase D antibody; Hexokinase type IV antibody; HHF3 antibody; HK IV antibody; HK4 antibody; HKIV antibody; HXKP antibody; MODY2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of GCK recombinant protein (Accession Number: NM_000162). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

  • Validations

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110905(GCK antibody) at dilution of 1:500

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110905(GCK antibody) at dilution of 1:500

    IP Result of anti-GCK (IP:Catalog No:110905, 4ug; Detection:Catalog No:110905 1:800) with mouse liver tissue lysate 4000ug.

    IP Result of anti-GCK (IP:Catalog No:110905, 4ug; Detection:Catalog No:110905 1:800) with mouse liver tissue lysate 4000ug.

  • Background
    Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism. The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.
  • References
    • Liu C, Lin JJ, Yang ZY, Tsai CC, Hsu JL, Wu YJ. Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells. Journal of agricultural and food chemistry. 62(48):11672-80. 2014.
    • Liu M, Pan Q, Chen Y. Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy. Scientific reports. 5:11219. 2015.
    • Yan HM, Xia MF, Wang Y. Efficacy of Berberine in Patients with Non-Alcoholic Fatty Liver Disease. PloS one. 10(8):e0134172. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"