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Product Name
GCC2 Polyclonal Antibody
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Description
Polyclonal antibody to GCC2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
GCC2 antibody; GCC185 antibody; RANBP2L4 antibody; REN53 antibody; GRIP and coiled-coil domain containing 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCC2 (NP_852118.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - GCC2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GCC2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - GCC2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using GCC2 antibody at dilution of 1:150 (40x lens).
Immunohistochemistry - GCC2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using GCC2 antibody at dilution of 1:150 (40x lens).
Immunohistochemistry - GCC2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human appendix using GCC2 antibody at dilution of 1:150 (40x lens).
Immunohistochemistry - GCC2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spleen using GCC2 antibody at dilution of 1:150 (40x lens).
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Background
Golgin which probably tethers transport vesicles to the trans-Golgi network (TGN) and regulates vesicular transport between the endosomes and the Golgi. As a RAB9A effector it is involved in recycling of the mannose 6-phosphate receptor from the late endosomes to the TGN. May also play a role in transport between the recycling endosomes and the Golgi. Required for maintenance of the Golgi structure, it is involved in the biogenesis of noncentrosomal, Golgi-associated microtubules through recruitment of CLASP1 and CLASP2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"