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Product Name
GC Polyclonal Antibody
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Description
Polyclonal antibody to GC
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
GC antibody; DBP antibody; DBP/GC antibody; GRD3 antibody; Gc-MAF antibody; GcMAF antibody; HEL-S-51 antibody; VDBG antibody; VDBP antibody; vitamin D-binding protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 324-493 of human GC (NP_001191236.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:10 - 1:100 -
Validations
Western blot - GC Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GC antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 120s.
Immunofluorescence - GC Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using GC antibody . Blue: DAPI for nuclear staining.
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Background
Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"