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GBP5 antibody

Cat.#: 110894

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Product Information

  • Product Name
    GBP5 antibody
  • Documents
  • Description
    GBP5 Rabbit Polyclonal antibody. Positive WB detected in U-937 cells, Jurkat cells. Positive IP detected in U-937 cells. Positive IHC detected in human prostate cancer tissue. Positive IF detected in MCF-7 cells. Positive FC detected in MCF-7 cells. Observed molecular weight by Western-blot: 65 kDa, 55 kDa
  • Tested applications
    ELISA, IHC, IF, WB, FC, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    GBP 5 antibody; GBP TA antigen antibody; GBP5 antibody; GTP binding protein 5 antibody; guanylate binding protein 5 antibody
  • Immunogen
    Human GBP5 Recombinant protein (GST tag)
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of GBP5 recombinant protein (Accession Number: NM_001134486). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    U-937 cells were subjected to SDS PAGE followed by western blot with Catalog No:110894(GBP5 Antibody) at dilution of 1:1500

    U-937 cells were subjected to SDS PAGE followed by western blot with Catalog No:110894(GBP5 Antibody) at dilution of 1:1500

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110894(GBP5 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110894(GBP5 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110894(GBP5 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110894(GBP5 antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of MCF-7 cells, using GBP5 antibody Catalog No:110894 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of MCF-7 cells, using GBP5 antibody Catalog No:110894 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    IP Result of anti-GBP5 (IP:Catalog No:110894, 4ug; Detection:Catalog No:110894 1:1000) with U-937 cells lysate 4000ug.

    IP Result of anti-GBP5 (IP:Catalog No:110894, 4ug; Detection:Catalog No:110894 1:1000) with U-937 cells lysate 4000ug.

    1X10^6 MCF-7 cells were stained with 0.2ug GBP5 antibody (Catalog No:110894, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 MCF-7 cells were stained with 0.2ug GBP5 antibody (Catalog No:110894, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    GBP5 represents guanylate binding protein 5
  • References
    • Meunier E, Dick MS, Dreier RF. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Nature. 509(7500):366-70. 2014.
    • Man SM, Karki R, Malireddi RK. The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection. Nature immunology. 16(5):467-75. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"