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Product Name
GATA1 antibody
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Description
GATA1 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HL-60 cells, NIH/3T3 cells, Raji cells. Positive IHC detected in mouse spleen tissue. Observed molecular weight by Western-blot: 55kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ERYF1 antibody; Erythroid transcription factor antibody; GATA 1 antibody; GATA binding factor 1 antibody; GATA1 antibody; GF 1 antibody; GF1 antibody; NF E1 DNA binding protein antibody; NFE1 antibody; XLTT antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GATA1 recombinant protein (Accession Number: BC009797). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:110878(GATA1 antibody) at dilution of 1:1200
Immunohistochemistry of paraffin-embedded mouse spleen slide using Catalog No:110878(GATA1 Antibody) at dilution of 1:50
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Background
GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].
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