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Product Name
GART antibody
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Description
GART Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells. Observed molecular weight by Western-blot: 108 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AIR synthase antibody; AIRS antibody; GAR transformylase antibody; GARS antibody; GART antibody; GARTF antibody; PAIS antibody; PGFT antibody; PRGS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GART recombinant protein (Accession Number: NM_175085). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110868(GART antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:110868(GART antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:110868(GART antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-GART (IP:Catalog No:110868, 4ug; Detection:Catalog No:110868 1:500) with HepG2 cells lysate 1600ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:110868(GART Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
GART represents phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
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