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Product Name
GANAB Polyclonal Antibody
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Description
Polyclonal antibody to GANAB
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
GANAB antibody; G2AN antibody; GIIA antibody; GLUII antibody; PKD3 antibody; neutral alpha-glucosidase AB antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human GANAB (NP_001265123.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - GANAB Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GANAB antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - GANAB Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using GANAB antibody at dilution of 1:150 (40x lens).
Immunohistochemistry - GANAB Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using GANAB antibody at dilution of 1:150 (40x lens).
Immunofluorescence - GANAB Polyclonal Antibody
Immunofluorescence analysis of C6 cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - GANAB Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - GANAB Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - GANAB Polyclonal Antibody
Immunofluorescence analysis of C6 cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - GANAB Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - GANAB Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"