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Product Name
GAMT antibody
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Description
GAMT Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, HEK-293 cells, human brain tissue, human heart tissue, mouse brain tissue, rat liver tissue. Positive IHC detected in human prostate cancer tissue, human liver tissue. Observed molecular weight by Western-blot: 26 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
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Alternative names
GAMT antibody; PIG2 antibody; TP53I2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GAMT recombinant protein (Accession Number: NM_000156). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:110860(GAMT antibody) at dilution of 1:6000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110860(GAMT antibody) at dilution of 1:10 (under 25x lens)
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Background
GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.
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References
- Wang L, Chen D, Yang L, Huang S, Zhang Y, Zhang H. Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio. Journal of fish biology. 76(5):1212-9. 2010.
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