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Product Name
Galectin 9 antibody
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Description
Galectin 9 Rabbit Polyclonal antibody. Positive WB detected in U-937 cells, HeLa cells, HepG2 cells, HL-60 cells, SGC-7901 cells. Positive IP detected in SGC-7901 cells. Observed molecular weight by Western-blot: 45kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Ecalectin antibody; Gal 9 antibody; Galectin 9 antibody; HUAT antibody; LGALS9 antibody; LGALS9A antibody; Tumor antigen HOM HD 21 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Galectin 9 recombinant protein (Accession Number: NM_002308). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
U-937 cells were subjected to SDS PAGE followed by western blot with Catalog No:110838(LGALS9, Galectin-9 Antibody) at dilution of 1:600
IP Result of anti-LGALS9, Galectin-9 (IP:Catalog No:110838, 4ug; Detection:Catalog No:110838 1:500) with SGC-7901 cells lysate 2400ug.
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Background
The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.
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References
- Yang J, Zhu L, Cai Y, Suo J, Jin J. Role of downregulation of galectin-9 in the tumorigenesis of gastric cancer. International journal of oncology. 45(3):1313-20. 2014.
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