Galanin (1-13)-Substance P (5-11) amide peptide

Galanin (1-13)-Substance P (5-11) amide peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

GWTLNSAGYLLGPQQFFGLM

H-Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2

20

97.7%

C₁₀₄H₁₅₁N₂₅O₁₆S

2199.56

Synthetic

Powder

The chimaeric peptide galantide is a high-affinity ligand for galanin receptors in membranes from rat hypothalamus, hippocampus, and spinal cord. There it reversibly blocks the neuronal actions of galanin in a dose-dependent manner. The peptide also reverses the galanin-mediated inhibition of insulin release from mouse pancreatic islets. Antagonists such as galantide are therefore of potential importance both as therapeutic agents and in studies on the physiological roles of galanin.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• T.Bartfai et al., Proc. Natl. Acad. Sci. USA, 88, 10961 (1991)
• S.Lindskog et al., Eur. J. Pharmacol., 210, 183 (1992)
• Ue.Langel et al., Int. J. Peptide Protein Res., 39, 516 (1992)
• T.Bartfai et al., Trends Pharmacol. Sci., 13, 312 (1992)
• D.Wynick et al., Proc. Natl. Acad. Sci. USA, 90, 4231 (1993)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.