Galanin (1-13)-Bradykinin (2-9) amide, M35 peptide

Galanin (1-13)-Bradykinin (2-9) amide, M35 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GWTLNSAGYLLGPPPGFSPFR

H-Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-NH2

21

97%

C₁₀₇H₁₅₃N₂₇O₂₆

2233.56

Synthetic

Powder

This galanin antagonist, which binds with high specificity and affinity to galanin binding sites, has been administered to rats to test the functional consequences of the galanin receptor blockade in a spatial learning task. The results suggest that galanin antagonists might provide a new principle in the treatment of dementia and related disorders.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• T.Bartfai et al., Trends Pharmacol. Sci., 13, 312 (1992)
• S.O.Oegren et al., Neuroscience, 51, 1 (1992)
• K.Kask et al., Regul. Peptides, 59, 341 (1995)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.