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GADD45A antibody

Cat.#: 110820

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Product Information

  • Product Name
    GADD45A antibody
  • Documents
  • Description
    GADD45A Rabbit Polyclonal antibody. Positive IP detected in K-562 cells. Positive WB detected in PC-13 cells, A549 cells, COLO 320 cells, K-562 cells, mouse lung tissue, mouse spleen tissue, rat lung tissue. Observed molecular weight by Western-blot: 18 kDa or 36 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DDIT 1 antibody; DDIT1 antibody; GADD45 antibody; GADD45A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of GADD45A recombinant protein (Accession Number: NM_001924). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

  • Validations

    PC-13 cells were subjected to SDS PAGE followed by western blot with Catalog No:110820(GADD45A Antibody) at dilution of 1:600

    PC-13 cells were subjected to SDS PAGE followed by western blot with Catalog No:110820(GADD45A Antibody) at dilution of 1:600

    IP Result of anti-GADD45A (IP:Catalog No:110820, 4ug; Detection:Catalog No:110820 1:500) with K-562 cells lysate 3200ug.

    IP Result of anti-GADD45A (IP:Catalog No:110820, 4ug; Detection:Catalog No:110820 1:500) with K-562 cells lysate 3200ug.

  • Background
    GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~36 kDa) and Mono (~18 kDa) forms (PMID:11498536).
  • References
    • Zhou J, Bi H, Zhan P. Overexpression of HP1γ is associated with poor prognosis in non-small cell lung cancer cell through promoting cell survival. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 35(10):9777-85. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"